Analysis Note

ControlRabbit IgG recombinant protein

Application

Sheep Anti-Rabbit IgG Antibody, HRP conjugate validated for use in western blotting.

General description

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules. This antibody is processed from Cat. No. AP510, Sheep anti-Rabbit IgG.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

StabilZyme is a registered trademark of SurModics, Inc.

Physical form

Purified sheep polyclonal in buffer containing PBS, StabilZyme^® HRP Conjugate Stabilizer, and 0.05% ProClin™ 150.

Quality

Evaluated by Western Blot in Rabbit IgG recombinant protein.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Rabbit IgG in 0.5 µg of Rabbit IgG recombinant protein.

Specificity

This antibody reacts with rabbit IgG and rabbit IgG (Fc). There may be minimal cross-reactivity against Human IgG and Rabbit IgM, with minimal to no cross-reactivity against Mouse IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG.

Target description

~53 kDa observed